Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:HeLa cells and Hodgkin’s Lymphoma,
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide.
Specificity：Recognizes the human Bax protein at 21kD. Does not react with Bcl-2a or Bcl-X protein. Species Crossreactivity: Does not recognize mouse and rat.
Clone # USB：2D2
Isotype：IgG1,k
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa 3-16,Cys-GSGEQPRGGGPTSS of human bax protein.
