The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression.
The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta.
The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein).
The E1A and E1B regions together comprise the transforming region of adenovirus.
While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region.
Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
Applications: Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry (frozen & formalin/paraffin).
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at RT for 20 min.
Recommended Dilution:Immunoprecipitation: Use Protein A; 2ug antibody/mg protein lysate)
Western Blotting: 1ug/ml
Immunohistochemistry: 2-4ug/ml
Optimal dilutions to be determined by the researcher.
仕様
Size：500ul
Host：mouse
Grade：Highly Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide.
