Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (formalin/paraffin sections): 1:200 for 20 min at RT.
No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.
Positive Control:Skeletal or Cardiac muscle, or Rhabdomyosarcoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation from ascites.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See A0760-24X.
Specificity：Recognizes human alpha-skeletal and alpha-cardiac (sarcomeric) actins as well as neoplasms derived from normal tissues (i.e. rhabdomyosarcomas). Shows no crossreaction with smooth muscle actin. Species Crossreactivity: bovine, human, rabbit, sheep, rat, guinea pig and frog.
Clone # USB：0.N.4
Isotype：IgM,k
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Bo Fr Gp Hu Rb Rt Sh
Immunogen：N-terminal decapeptide of alpha skeletal muscle isoform of actin; acetylated at the N-terminus. Cellular Localization: Cytoplasmic. Epitope: N-terminal decapeptide.
