Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point.
Anti-Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups.
Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive.
Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.
This antibody is useful in the identification of rhabdoid cellular elements.
Applications:Suitable for use in Immunohistochemistry.Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 minutes at RT.
No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilution to be determined by the researcher.
Positive Control:Muscle
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Supernatant
Form：Supplied as a liquid, 0.09% sodium azide.
Specificity：Recognizes human actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart, and smooth muscle cells. Recognizes alpha actin from skeletal, cardiac, and smooth muscle, and gamma actin from smooth muscle sources. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). Species Crossreactivity: rabbit and rat.
Clone # USB：0.N.3
Isotype：IgG1,k
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Rb Rt
Immunogen：SDS extract of human myocardium. Cellular Localization: Cytoplasmic
