Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP).
Although the exact biochemical function of BAP is uncertain, measurement of the serum levels of BAP can be used as a biochemical indicator of bone turnover.
Conditions which present with a decrease in the level of BAP (hypophosphatasemia) include the inherited bone-deforming disorder hypophosphatasia and osteoporosis, while an increase in BAP (hyperphosphatasemia) is associated with Paget’s disease, bone fractures and osteosarcomas.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes Alkaline Phosphatase from human bone. Crossreactivity: Alkaline phosphatase from human placenta, calf intestine and bacterial.
Clone # USB：0.G.2 (BGN/03/662)
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Purified alkaline phosphatase (native) from human bone.
