85-7206-81　TCF-4 (T-cell Transcription Factor 4) 200ug　T2026

特徴

• TCF-4, transcription factor 4, is a basic helix-turn-helix transcription factor.
• This protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG')-a motif first identified in immunoglobulin enhancers.
• The gene for TCF-4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well.
• Multiple alternatively spliced transcript variants that encode different proteins have been described.
• TCF4 is expressed widely during development.
• Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine.
• TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages.
• Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.
• Applications:Suitable for use in Western Blot, ELISA, Supershift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/ml.
• Detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4.
• Supershift Assay: Reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA).
• Immunoprecipitation: Reported to immunoprecipitate TCF-4 from SW620 whole cell lysates as a complex with b-Catenin.
• Immunocytochemistry/Immunohistochemistry: Reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS.
• Optimal dilutions to be determined by the researcher.
• Positive Control:DLD1 (human colorectal adenocarcinoma), Cos-1 cells expressing transfected TCF-4 or HCT116 (human colorectal carcinoma) cells.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap