85-7206-79　［取扱停止］IQGAP2 200ul　I8448-76

特徴

• This gene encodes a member of the IQGAP family.
• The protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain.
• It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility.
• [provided by RefSeq]
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:2000.
• HepG2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with I8448-76 (1:2000).
• Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system.
• Immunoprecipitation: 4ug immunoprecipitated IQGAP2 from 500ug of HepG2 RIPA lysate.
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
• I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.