85-7206-77　STAT 5a,b, phosphorylated (Tyr694/Tyr699) (Signal Transducer and Activator of Transcription 5a,b) 100ug　S7972-13

特徴

• STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases.
• In resting cells, the STATs exist in an inactive state in the cytoplasm until activated.
• Activators include cytokines, growth factors, and some peptides.
• These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT.
• Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers.
• STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand (1).
• After dimerization, the STATs accumulate in the nucleus by way of nuclear transport.
• STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production.
• The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000 detected phospho-STAT5A/B in A431 cells stimulated with EGF.
• The lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with S7972-13.
• Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Optimal dilutions to be dtermoned by the researcher.
• Positive Control:
• IFN-alpha induced HeLa cells, GM-CSF treated TF-1 cells.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.