85-7206-76　FADD (Fas-Associated Death Domain Protein, MORT1) 100ug　F0019-51

特徴

• FADD was originally isolated as a protein that bound to the cytoplasmic domain of Fas in the yeast two-hybrid system.
• Sequence analysis revealed a region homologous to the death domain of Fas and TNFR-1.
• Subsequent biochemical studies have shown that FADD associates with Fas through interaction of the death domains.
• When over expressed in several cell lines, FADD induces apoptosis, which can be blocked by CrmA, an inhibitor of Caspase 1 (ICE).
• This evidence suggests that FADD plays a role in Fas-mediated apoptosis.
• Applications:Suitable for use in Western Blot.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/ml, detects FADD in RIPA lysated from Jurkat cells
• Optimal dilutions to be determined by researcher.
• Positive Control (included):J9000: Jurkat Cell
• Hybridoma:Fusion of mouse myeloma P3U1 with Balb/c splenocytes.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.