85-7206-75　STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) 50ug　S7971-06

特徴

• Stat3 is a key signaling molecule for many cytokines and growth-factor receptors, and is required for murine fetal development.
• Additionally, Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities.
• Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding.
• Transcriptional activation seems to be regulated by phosphorylation at Ser727 via the MAPK or mTOR pathway.
• Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3a (86 D) and Stat3b (79kD) depend on cell type, ligand exposure or maturation stage of the cells.
• It is notable that Stat3b lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-1ug/ml.
• Detects phospho-STAT3 (Tyr705) in lysates from human SK-N-MC cells stimulated with CNTF.
• Immunoprecipitation (Reported): 1-10ug/ml immunoprecipitates phospho-STAT3 (Tyr705) from a lysate of 10e6 vanadate-treated human A431 cells.
• Optimal dilutions to be determined by reseacher.
• Recommended Antibodies of Choice:Goat anti-mouse IgG labeled with horseradish peroxidase (HRP)
• I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
• I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo (does not crossreact with rat)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.