85-7206-72　Eck (EphA2, Mpk-5, Native Epithelial Cell Kinase, Eph receptor tyrosine kinase A2) 200ug　E3360-01B

特徴

• Applications:Suitable for use in Western Blot, Protein Kinase Assay, Immunoprecipitation and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/ml; Detected Eck in RIPA lysates from human A431 cells and previously from foreskin fibroblasts, murine 3T3/A31 and rat L6 cells.
• A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E3360-01B.
• Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system
• Protein Kinase Assay: Reported to have been used in an IP autophosphorylation assay, using a Mn-PIPES reaction buffer.
• Immunoprecipitation: Reported to immunoprecipitate Eck from 500ug of a human breast epithelial cell line which had been lysed in TBS containing 1% Triton X-100.
• Use 1-4ug per reaction.
• Immunocytochemistry: Reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7% formaldehyde solution and permeabilized with 0.5% Triton X-100 in TBS.
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibody:I1904-77B: IgG1 (HRP) Pab Gt x Mo
• Positive Antigen Control:A0001-50: Non-stimulated A431 lysate
• Control cells:CHO cell lysate, foreskin fibroblasts, 3T3/A31 cells, L6 cells.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.