85-7206-70　GABA A Receptor beta 2,3 200ug　G1016

特徴

• GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters.
• They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A).
• Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics.
• GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma).
• The subunits determine the pharmacological characteristics.
• Applications:Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/ml detected the GABAA Receptor beta 2,3 subunits from 20ug of rat brain microsomal preparation.
• Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G1016 (2ug/ml).
• Proteins were visualized using a IgG (HRP) GtxMo and ECL.
• Immunoprecipitation: 4ug immunoprecipated the GABAA Receptor beta 2,3 subunits from 500ug of rat brain microsomal preparation.
• Due to interference of the IgG heavy chain, the immunoprecipitated GABAA reseptor complex was confirmed using G1015-06.
• Immunohistochemistry: Detects GABAA Receptor beta 2,3 subunits in paraffin embedded and frozen rat brain sections fixed with 4% paraformaldehyde or 0.1% glutaraldehyde and permeabilized with Triton X-100.
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
• I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.