85-7206-69　［取扱停止］FceRI, alpha (Fc epsilon RI, IgE Receptor) 200ug　F0025

特徴

• The allergic reaction is mediated through the IgE high affinity receptor (FceRI).
• When a multivalent antigen is presented, a redistribution of the monomeric IgE-FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction.
• The FceRI receptor is a tetrameric complex, consisting of an a-chain, a b-chain and a dimeric g-chain.
• While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases.
• The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.
• Applications:Suitable for use in Immunoprecipitation/Western Blot and FACS.
• Not suitable for Western Blot without pre-Immunoprecipitation of antibody.
• Other applications have not been tested.
• Recommended Dilutions:Immunoprecipitation: 5ug immunoprecipitates rat FceRI alpha from 500ug of biotinylated rat basophilic leukemia cell lysate.
• The immunoprecipitate was resolved by electrophoresis and transferred to nitrocellulose.
• Biotinylated protein bands were visualized using NeutrAvidin labeled with HRP and an enhanced chemiluminescence detection system.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.