85-7206-66　Histone H1 discontinued 200ug　H5110-01

特徴

• Histones are a family of basic proteins found in all eukaryotic nuclei that are associated with eukaryotic DNA.
• The five major types of histone are H1, H2A, H2B, H3 and H4.
• The latter four, which comprise nucleosome core, are evolutionarily conserved, while the linker histone H1 exhibits greater sequence diversity among organisms.
• The amino acid sequence of H1 varies more from organism to organism.
• The separation of H1 into multiple bands by gel electrophoresis results from differences in the extent of phosphorylation of residues in the protein.
• This antibody can be used as a control for H5110-03.
• Application:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1-4ug/ml detects histone H1 in lysates from an acid extract of HeLa cells.
• Lysate from acid extracted HeLa cells was resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-01.
• Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
• Immunocytochemistry: 5-20ug/ml showed positive nuclear immunostaining in A431 cells fixed with 4% paraformaldehyde and methanol.
• Optimal dilutions to be determined by researcher.
• Recommended Secondary Antibodies (WB):I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
• I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
• Recommended Secondary Antibodies (IC):I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt x Mo
• Positive Control:HeLa, NIH3T3 cells
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.