85-7206-64　Cbl 200ug　C2097-95

特徴

• Casitas B-lineage lymphoma (Cbl), is an oncogene which causes pre B-cell lymphomas.
• It participates in the regulation of cell signaling and has the ability to interact with many proteins such as receptor/non-receptor PTK's, structural proteins, and novel proteins that are not well understood.
• Diseases associated with Cbl are autoimmune diseases, diabetes, and leukemia.
• By modifying Cbl's ability to bind to oncogenic proteins, Cbl may be developed into an anti-cancer tool and in the near future used in therapeutic intervention.
• Applications:Suitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/ml detects Cbl (120kD) in RIPA lysates from human Jurkat and mouse 3T3 cells.
• 3T3 cell lysate was resolved by electro-phoresis, transferred to nitrocellulose and probed with anti-Cbl (1ug/ml).
• Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunoprecipitation: 2ug of a previous lot immunoprecipitated Cbl from 1ug of a mouse 3T3 cell RIPA lysate.
• Immunocytochemistry: 5-10ug/ml of a previous lot showed positive immunostaining for Cbl in 3T3 cells fixed with ice cold methanol/ethanol [1:1] for 1 minute.
• Optimal dilution determined by the researcher.
• Positive Control Included:F4209: 3T3 lysate.
• Use 20ug per lane for minigels.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.