85-7206-63 [取扱停止]Lck, p56 (Lymphocyte-specific Tyrosine Kinase) 100ug L1565
特徴
- Western Blot: 0.5–2mg/ml detects Lck in RIPA lysates from Jurkat cells.
- Immunoprecipitation Kinase Assay: Reported to immunoprecipitate enzymatically active Lck as determined by immune complex kinase assays.
- Immunoprecipitation: 3mg of a previous lot immunoprecipitated Lck from 1mg of Jurkat RIPA lysate.
- Positive Antigen Control:Jurkat cell lysate.
- Use 20ug per lane for minigels.
- Jurkat cell lysate was resolved by electro-phoresis, transferred to nitrocellulose and probed with anti-Lck (0.5mg/ml).
- Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
- Storage and Stability:May be stored at 4°C for short-term only.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Aliquots are stable for 12 months.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size:100ug
- Host:mouse
- Source Antibody:mouse
- Grade:Affinity Purified
- Purity:Purified by Protein A affinity chromatography.
- Form:Supplied as liquid in 10mM PBS, pH 7.4.
- Specificity:Recognizes and is specific for Lck at 56kD. Species Crossreactivity: mouse, human and rat.
- Clone # USB:3A5
- Isotype:IgG2b,k
- Calc Applications Abbrev:IP WB
- Calc Crossreactivity:Hu Mo Rt
- Immunogen:Fusion Protein corresponding to aa1–225 of mouse Lck.
- Shelf Life:1year
- EU Commodity Code:30021010
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