NR1 along with the NR2 subunit forms a heterodimer that make up the NMDA receptor.
The NMDA receptor is characterized as a ionotropic receptor for glutamate.
As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor.
A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium.
Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations.
The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell.
Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
Applications:Suitable for use in Immunocytochemistry, Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry: Shows positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.
Immunoprecipitation: 4ug immunoprecipitated NR1 from 500ug of rat brain microsomal protein preparation.
Western Blot: 0.5-2ug/ml.
Detects NR1 from 20ug of rat brain microsomal protein preparation.
Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with N5380-05 (0.5ug/ml).
Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Note: Do not boil the microsomal preparation.
Incubate at RT for 30-45 minutes.
Optimal dilutions to be determined by the researcher.
Positive Control:
Brain tissue
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：mouse
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes rat NR1 (Mr 130kD). An additional band (Mr 200kD) may be detected at higher concentrations. The epitope maps to a region (aa834-864) common to all NR1 isoforms and so should crossreact with all NR1 isoforms.
Clone # USB：0.T.190
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Rt
Immunogen：His tag fusion protein corresponding to aa834-938 of rat NR1. Peptide sequence homology: human and mouse, 100%
