85-7206-60　Myelin Basic Protein, phosphorylated (MBP) 50ug　M9758-05

特徴

• Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich.
• Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath.
• MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles.
• MBP serves as a marker for oligodendrocytes and Schwann cells.
• MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.
• Applications:Suitable for use in Western Blot, Protein Kinase Assay and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 0.5-2ug/ml.
• Strongly detects phosphorylated MBP.
• Note: Some preparations of MBP contain basal levels of phosphorylated MBP, which are detected by the M9758-05.
• Protein Kinase Assay: Phosphorylated MBP was detected in a direct kinase assay using non-radioactive MAP Kinase Assay Kit, BioAssay™ (M2353) and usign MAP Kinase Immunoprecipitation Kinase Assay Kit, BioAssay™ (M2353-50).
• Immunocytochemistry: Reported by an independent lab (3).
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.