85-7206-57　Paxillin (PXN) 250ug　P3113-90

特徴

• Paxillin can be diffusely distributed in the cytoplasm, colocalized with actin at the cell periphery, or aggregated at focal adhesions.
• Paxillin has several motifs which allow its interaction with other proteins.
• These motifs include LD motifs, LIM domains, an SH3 domain binding site, and SH2 domain binding sites.
• Paxillin complexes with proteins of focal adhesions including vinculin, talin, with the kinases FAK, PYK2, Src, and Csk, with ARF GTPase activating proteins, and with the adaptor protein v-Crk.
• Paxillin is phosphorylated at several residues, including tyrosine 31, tyrosine 118, tyrosine 181.
• The phosphorylation of paxillin appears to be important for several biological functions including S-phase transition and neurite extension.
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/ml detects paxillin in RIPA lysates from 3T3 fibroblasts using 20ug per lane for minigels.
• Immunoprecipitation: 4ug per 500ug of cell lysate.
• Immunocytochemistry:5-10ug/ml (gave positive immunostaining for paxillin in REF (rat embryo fibroblast) 52 cells fixed with 3.7% paraformaldehyde for 5 minutes).
• Optimal dilutions to be determined by the researcher.
• Positive Antigen Control:Add 2.5ul 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
• Load 20ug of reduced lysate per lane for immunoblot analysis.
• For use as a positive control for antibodies.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.