Vinculin, a cytoskeletal protein found in adherens type cell-cell and cell-extracellular matrix junctions, displays considerable heterogeneity.
At least three 117kD and two 130kD isoforms (muscle specific metavinculin) have been detected.
Vinculin binds talin and a-actinin in adhesion plaques and may play either a structural or a regulatory role in these structures.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-1ug/ml detects Vinculin in RIPA lysates of human A431 cells; previous lots detected Vinculin in RIPA lysates from chicken embryonic fibroblasts (CEF) and mouse 3T3 fibroblasts.
Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with V2122-50 (0.5ug/ml).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates Vinculin from mouse 3T3/A31 and human A431 RIPA lysates.
Immunohistochemistry and Immunocytochemistry (frozen and formalin fixed sections): 10ug/ml will stain Vinculin in Ref-52 cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100.
Optimal dilutions to be determined by the researcher.
Positive Antigen Control (Included):A0001-50, Non-stimulated A431 cell lysate.
Use 20ug per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 1mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes human vinculin at 117kD. Species Crossreactivity: rat, mouse, rabbit and chicken.
