85-7206-51　Actinin, alpha 1 (Non-muscle) 200ug　A0761

特徴

• The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions.
• Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors.
• Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry.
• Not suitable for use in Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/ml.
• Detects alpha-Actinin in RIPA lysates from human A431 cells.
• Immunocytochemistry/Immunohistochemistry: 15ug/ml.
• Reported positive immunostaining for alpha-Actinin in MRC-5 cells fixed with methanol and also in frozen sections.
• Optimal dilutions to be determined by the researcher.
• Positive Antigen Control (Included):A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.
• Recommended Secondary Antibodies:I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
• I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
• I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt x Mo
• I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt x Mo
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.