E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter.
The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity.
E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Electrophoretic Mobility Shift Assay.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml of antibody detected E2F-1 in a HeLa nuclear extract.
Immunoprecipitation: 4ug immunoprecipitated E2F-1 from HeLa nuclear extract.
Optimal dilutions to be determined by the researcher.
Positive Antigen Control (Included):H1838: HeLa Nuclear Extract
Hybridoma:SP2 mouse myeloma cells with immunized mouse splenocytes.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human E2F-1 at ~55kD. Species Crossreactivity: Xenopus
Clone # USB：KH20 and KH95
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Xe
Immunogen：GST fusion protein corresponding to full-length human E2F-1. Epitope: aa1-89 for clone KH20; aa342-386 for clone KH95.
