Applications:Suitable for use in Immunoblot and Immunoprecipitation.
Other applications not tested.
Recommended DilutionWarren
Immunoblot: 0.5-5ug/ml detects cyclin B1 in RIPA lysates from human A431 carcinoma cells.
Immunoprecipitation: 2ug precipitated human cyclin B1 and cdc2 kinase from 500ug of A431 RIPA lysate.
Optimal dilutions to be determined by the researcher.
Positive Antigen Control: A0001-50
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Specific for human cyclin B1 (~ 58kD). Does not crossreact with cyclin A or cyclin D. Species crossreactivity: Human, weak species crossreactivity with mouse was observed..
Clone # USB：0.T.183 (GNS3 (8A5D12))
Isotype：IgG2b
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human cyclin B1.
