85-7206-46　ATPase alpha 1, Na, K 50ug　A4000-52

特徴

• Na+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase).
• It is found in the plasma membrane of virtually every human cell and is common to all cellular life.
• It helps maintain cell potential and regulate cellular volume.
• In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular).
• Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane.
• In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 0.05-0.1ug/ml detected the a-1 subunit in 20ug of rat brain microsomal preparations using chemiluminescence.
• To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.
• Immunohistochemistry: 1-10ug/ml on ice-cold methanol-fixed tissue.
• Immunoprecipitation: 2ug immunoprecipitated the a-1 subunit from rat brain microsomal preparations.
• Optimal dilutions to be determined by the researcher.
• Source:Culture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.