85-7206-41　NFkB, p52 (NF Kappa B, NF-kB, Nuclear Factor kappa B) 200ug　N2301-60

特徴

• The NFkB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein.
• The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68.
• The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105.
• A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkB.
• It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFkB subunit, in this case a protein designated p52 (previously referred to as p49).
• In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Gel Shift Assay.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/ml.
• Detects NFkB p52 and its p100 precursor in HeLa nuclear extract and human Raji cell but not in mouse 3T3 cell and rat PC 12 cell lysates.
• Immunoprecipitation: 4ug immunoprecipitates NF-kB p52 and its p100 precursor from 500ug HeLa nuclear extract cell lysate.
• Gel Shift Assay: Use 2ug per assay
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
• I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
• Positive Control Included:H1838: HeLa nuclear extract.
• Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation.
• Load 20ug of reduced lysate per lane for minigels.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.