85-7206-39　Fas (CD95, APO-1) 200ug　F0019-62

特徴

• Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms.
• This “cellular suicide” can be mediated by the Fas antigen (CD95, APO1), a cell-surface glycoprotein, 40-50kD, that belongs to the nerve growth factor/tumor necrosis factor (TNF) receptor family.
• Mice carrying the lymphoproliferation (lpr) mutation, which develop lymphoadenopathy and suffer from a systematic lupus erythematosus-like autoimmune disease, have defects in the Fas gene.
• Fas is expressed in several tissues including blood, where it expression is upregulated on activated T and B lymphocytes and natural killer cells.
• Binding of the Fas antigen by agonistic anti-Fas antibody has been shown to induce apoptosis.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/ml detects Fas (~45 kD) in RIPA lysates of mouse 3T3/A31 fibroblast cells and bovine brain.
• Immunoprecipitation: 4ug immunoprecipitates Fas from 3T3/A31 cells.
• RIP, a death domain containing protein, was co-precipitated with Fas.
• Optimal dilutions to be determined by the researcher.
• Positive Antigen Control:F4209: 3T3/A31 Cell Lysate.
• Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
• Use 20ug per lane for minigels.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.