85-7206-36　MAP2 (Microtubule Associated Protein 2) 200ug　M2350-02

特徴

• MAP2 is a type II microtubule associated protein (MAP).
• It is located predominately in dendrites where it accelerates tubulin polymerization, cross-links microtubule fibers into stable bundles, and connects microtubule filaments to intermediate filaments.
• Alternative splicing of MAP2 mRNA gives rise to at least three different isoforms including; MAP2a (280kD), MAP2b (200kD), and MAP2c (70kD).
• The MAP2c isoform is expressed in embryos, whereas the MAP2a and MAP2b isoforms are expressed in adult tissues.
• MAP2 is the somatodentritic MAP of neurons, but it also is expressed to a lesser extent in glia.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1-2ug/ml detects MAP2 in 20ug of rat brain microsomal protein preparation.
• Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with M2350-02 (0.5ug/ml).
• Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
• Immunohistochemistry: 10ug/ml detects MAP2 in rat brain sections.
• Optimal dilutions to be determined by the researcher.
• Recommended secondary antibodies:I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
• I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
• I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
• I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.