85-7206-34　Fas, Neutralizing (CD95, APO-1) 100ug　F0019-64

特徴

• Fas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family.
• Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell.
• The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD.
• FADD facilitates binding of caspase-8.
• which is activated to induce caspase-8 mediated apoptosis.
• Fas also plays a role in T cell mediated cytotoxicity and T cell development.
• Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes.
• Applications:Suitable for use in Neutralization, Flow Cytometry and Western Blot.
• Other applications have not been tested.
• Recommended Dilutions:Neutralization: 10-500ng/ml of this antibody was incubated for one hour with human Fas- transfected cells and inhibited apoptosis induced by the anti-Fas (human, activating) clone CH11/clone 0.T.45 (Catalog # F0019-63) treatment of the cells.
• Cell viability was assessed using a WST-1 assay.
• Western Blot: 5ug/ml
• Flow Cytometry: 5-20ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.