Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades.
Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily.
To date, approximately 90 members of this superfamily have been identified in the human genome.
Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli.
Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml.
Detects tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells.
Immunoprecipitation: 2-4ug.
Immunoprecipitates quantitatively the phosphotyrosine-containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase.
To preserve phosphotyrosine, add 0.2mM sodium orthovanadate to the lysis buffer.
