85-7206-25　Nitrotyrosine (nTyr) 100ug　N2700-05

特徴

• Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO.
• It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell.
• Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.
• Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/ml detects nitrated proteins in a lysate prepared from peroxynitrite A431 cells.
• A previous lot detected nitrotyrosine immunoblotting controls.
• A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells.
• No reaction was noted with non-nitrated proteins.
• Immunocytochemistry: 5-10ug/ml showed positive immunostaining for nitrated proteins in peroxynitrite-treated (5-10mM for 5 min) A431 cells fixed with 95% ethanol/5% acetic acid.
• Optimal dilutions to be determined by the researcher.
• Recommended Controls:Immunohistochemistry: Human Alzheimer diease brain sections (Methacarn fixed), Mouse livers
• Western Blot: Nitrated-BSA
• Indirect ELISA: Nitrated-BSA
• Indirect Immunofluorescence: A549 cells metabolically labeled with 3-nitrosine
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.