Src is a member of the Src family of protein tyrosine kinases.
This kinase is expressed in different tissues of the body with the highest protein levels detected in neurons and platelets.
Src can modulate the signal transduction pathways activated by several growth factors (e.g., PDGF, M-CSF and G-CSF) and integrins.
Src also regulates the activity of several ion channels including the N-methyl-D-aspartate (NMDA) receptor.
In addition, Src is thought to play a role in physiological/ pathophysiological processes in the central nervous system.
It has also been reported that mice deficient in Src develop severe osteoporosis.
A total of eight membrane-associated protein tyrosine kinases have been identified within the Src gene family.
Members of this group include c-Src, c-Yes, Fyn, Lck, Lyn, Blk and c-Fgr whose roles include regulation of cytoskeletal organization, cell-cell contact, DNA synthesis, proliferation and differentiation of eukaryotic cells.
Elevated activity of the proto-oncogene Src has been found in many human cancers, most notably breast cancer.
The highest levels of expression of the prototype member of this gene family, Src p60, are observed in platelets and in neuronal tissues.
With the fact that platelets have constitutively high levels of phosphotyrosine-containing proteins suggest that Src p60 may be important for platelet homeostasis.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-5ug/ml detected Src (60kD) in a RIPA lysate of chicken embryo fibroblasts (CEF).
Immunoprecipitation: 1-2ug immnoprecipitated Src using 250-500ug of Src-transformed chicken cell extract, as reported by an independent laboratory.
Immunocytochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：mouse
Source Antibody：chicken
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes and is specific only for avian Src at ~60kD. This antibody is excellent for distinguishing pp60src in cells transfected with v-src or c-src expression vectors.
Clone # USB：0.T.138 (EC10)
Isotype：IgG2b
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Av Ch
Immunogen：Chicken Src, purified from extracts of E. coli containing the plasmid plac-src.
