85-7206-18　［取扱停止］FAK (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) 100ug　F0019-55

特徴

• FAK is a widely expressed non-receptor protein tyrosine kinase (PTK).
• FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors.
• Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin.
• Studies have shown that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase.
• In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin.
• In vitro the Grb2 SH2 domain binds directly to tyrosine- phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.
• Applications:Suitable for use in Immunoprecipitation and Immunofluorescence.
• Not recommended for Western Blot.
• Other applications have not been tested.
• Recommended Dilutions:Immunofluorescence: 10ug/ml
• Immunoprecipitation/Western Blot: 4ug immunoprecipitates FAK from 500ug of a mouse 3T3/A31 cell RIPA lysate.
• The immunoprecipitate was resolved by electrophoresis, transferred to nitrocellulose and probed with F0019-56 (2ug/ml).
• Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system.
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
• I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
• I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.