85-7206-10　Phospholipase C, gamma 1 (PLCg1) 50ug　P4074-42

特徴

• Phospholipase C gamma 1 is a tryosine kinase substrate for many receptor and nonreceptor tyrosine kinases.
• Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-triphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C.
• Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity.
• PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLC gamma activity is unknown.
• Applications:Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 0.5-2ug/ml detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations.
• Immunoprecipitation: 4ug Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation.
• Immunocytochemistry: 5-10ug/ml detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells.
• Optimal dilutions to be determined by the researcher.
• Positive Antigen Control (Included):A0001-50 (Non-stimulated A431 cell lysate).
• Add 2.5ul of 20mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
• Use 20ug per lane for minigels.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.