Basic fibroblast growth factor (bFGF or FGF2) is a member of the fibroblast growth factor family.
In normal tissue, bFGF is present in basement membranes and in the subendothelial extracellular matrix of blood vessels.
It stays membrane-bound as long as there is no signal peptide.
It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels.
Additionally, bFGF is a critical component of human embryonic stem cell culture medium.
Applications:Suitable for use in Neutralization.
Not suitable for Western Blot, use F4210-21 Fibroblast Growth Factor 2, Basic (FGF2b).
Other applications have not been tested.
Recommended Dilutions:Neutralization of bFGF Activity Assay: 2.5ug/ml neutralizes >40% growth of bovine FBHE cells simulated with 5ng/ml of FGF-2/bFGF.
Optimal dilutions to be determined by the researcher.
Hybridoma:P3X63-Ag8.U1 myeloma cells with spleen cells from Balb/c mice.
Recommended Positive Control:Fetal liver or kidney tissue
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：mouse
Source Antibody：bovine
Grade：Highly Purified
Purity：Purified by ammonium sulfate precipitation and DEAE-cellulose chromatography from ascites.
Form：Supplied as a liquid in TBS. No preservative added.
Specificity：Recognizes the non-denatured form of bovine basic FGF2. Does not crossreact with bovine acidic FGF (HBGF-1). Species Crossreactivity: human, rat and mouse.
