Neutralization of Biological Activity: 10ug/ml causes a 74% reduction in cell number in a 5 day cell proliferation assay using A431 cells.
Western Blot: 0.5-2ug/ml detects non-reduced EGF receptor in RIPA lysates from non-stimulated human A431 cells.
Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E3375-14 (1ug/ml).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates the EGF receptor from 500ug of non-stimulated A431 RIPA lysate.
Immunocytochemistry: 10ug/ml shows positive immunostaining for the EGF receptor in A431 cells fixed with 4% para-formaldehyde.
Optimal dilutions to be determined by the researcher.
Biological Activity:Inhibits A431 cell growth.
Recognizes an antigenic determinant located on the extracellular domain of the receptor.
Positive Antigen Control:A0001-50: A431 Cells, Non-Stimulated Use 20ug per lane for minigels.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：250ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a lyophilized powder from 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride. Reconstitute with 250ul sterile ddH2O. Further dilute with PBS, 5mg/ml BSA.
Specificity：Recognizes the external domain of EGF receptor on all human cells at ~180kD. Competes with EGF and TGF-a for binding on human cells. Recognizes both phosphorylated and non-phosphorylated human EGF receptors. Does not react with EGF receptors on mouse or rat cells.
