85-7205-99　Phosphotyrosine (FITC) 100ug　P4078-09

特徴

• Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades.
• Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily.
• To date, approximately 90 members of this superfamily have been identified in the human genome.
• Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli.
• Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.
• Applications:Suitable for use in Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunocytochemistry: 5ug/ml.
• Detects phosphotyrosine containing proteins in EGF-stimulated human A431 cells.
• Optimal dilutions to be determined by researcher.
• Hybridoma:FOXY-NY (a NS-1 derivative) crossed with spleen cells from immunized mice.
• Positive Control:
• Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells
• Storage and Stability
• Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.