Applications:Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, and Chimerism Studies.
Other applications have not been tested.
Recommended Dilution:Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution.
Optimal dilutions to be determined by the researcher.
Cytotoxicity:Expected Results:Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody.
Live lymphocytes indicate a negative reaction.
Dead lymphocytes indicate a positive reaction.
Limitations:Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at 4°C.
Aliquots are stable at 4°C for 12 months after receipt.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ul
Host：mouse
Source Antibody：human
Grade：Highly Purified
Purity：≥98%, Purified by Ion Exchange chromatography.
Form：Supplied as a liquid in PBS, 1% BSA., 0.09% sodium azide Labeled with Fluorescein isothiocyanate (FITC).
