The hepatitis C virus particle consists of a core of genetic material (RNA), surrounded by an icosahedral protective shell of protein, and further encased in a lipid (fatty) envelope of cellular origin.
Two viral envelope glycoproteins, E1 and E2, are embedded in the lipid envelope.
Hepatitis C virus has a positive sense single-stranded RNA genome.
The genome consists of a single open reading frame that is 9600 nucleotide bases long.
This single open reading frame is translated to produce a single protein product, which is then further processed to produce smaller active proteins.
At the 5' and 3' ends of the RNA are the UTR, that are not translated into proteins but are important to translation and replication of the viral RNA.
The 5' UTR has a ribosome binding site[3] (IRES-Internal ribosome entry site) that starts the translation of a very long protein containing about 3,000 amino acids.
The core domain of the hepatitis C virus (HCV) IRES contains a four-way helical junction that is integrated within a predicted pseudoknot.
The conformation of this core domain constrains the open reading frame's orientation for positioning on the 40S ribosomal subunit.
The large pre-protein is later cut by cellular and viral proteases into the 10 smaller proteins that allow viral replication within the host cell, or assemble into the mature viral particles.
Structural proteins made by the hepatitis C virus include Core protein, E1 and E2; nonstructural proteins include NS2, NS3, NS4, NS4A, NS4B, NS5, NS5A, and NS5B.
Applications:Suitable for use in ELISA.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：mouse
Grade：Affinity Purified
Purity：Purified by Protein G-Sepharose affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide before the addition of glycerol to 40%
Specificity：Recognizes aa80-160 epitope of HCV Core antigen.
Clone # USB：8.V.023
Isotype：IgG2a
Calc Applications Abbrev：E
Immunogen：Recombinant HCV core antigen consisting of aa80-160.
