特徴

• The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans.
• The superlocus contains a large number of genes related to immune system function in humans.
• This group of genes resides on chromosome 6, and encodes cell-surface antigen-presenting proteins and many other genes.
• The HLA genes are the human versions of the MHC genes that are found in most vertebrates (and thus are the most studied of the MHC genes).
• The proteins encoded by certain genes are also known as antigens, as a result of their historic discovery as factors in organ transplantations.
• The major HLA antigens are essential elements for immune function.
• Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell (including viral peptides if present).
• These peptides are produced from digested proteins that are broken down in the proteasomes.
• The peptides are generally small polymers, about 9 amino acids in length.
• Foreign antigens attract killer T-cells (also called CD8 positive- or cytotoxic T-cells) that destroy cells.
• Applications:This antibody has not been tested in methodologies other than Microcytotoxicity Test.
• Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.
• Recommended Dilutions:Cytotoxicity Working Dilution: Add 0.9ml of PBS, 1% BSA to 100ul of 10X stock solution.
• Optimal dilutions to be determined by the researcher.
• Cytotoxicity:Expected Results:Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody.
• Live lymphocytes indicate a negative reaction.
• Dead lymphocytes indicate a positive reaction.
• Limitations:Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
• Source:Ascites fluid
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.