85-7204-34　Factor X 100ug　F0018-05

特徴

• Bovine Factor X is a vitamin K-dependent protein zymogen which is synthesized in the liver and circulates in plasma as a two chain molecule linked by a disulfide bond (1,2).
• Prior to secretion into plasma, post-translational modifications produce 11 gamma-carboxyglutamic acid (gla) residues and a single b-hydroxyaspartic acid residue, which are located within the NH2-terminal light chain.
• The light chain also contains two epidermal growth factor (EGF) homology domains.
• The COOH-terminal heavy chain of factor X contains most of the carbohydrate moieties, as well as the latent serine protease domain.
• The activation of factor X is catalyzed by either the intrinsic factor X-ase complex (factor IXa, factor VIIIa, cellular surface and calcium ions) or the extrinsic factor X-ase complex (factor VIIa, tissue factor, cellular surface and calcium ions).
• Activation of bovine factor X by either complex results in cleavage at Arg52-Ile53 of the COOH-terminal heavy chain and subsequent release of a 52 amino acid activation glycopeptide.
• Factor Xa then serves as the enzyme component of the prothrombinase complex which is responsible for the rapid conversion of prothrombin to thrombin.
• The gla residues enable factor X/Xa to bind phospholipid (i.e.
• cell surfaces) in a calcium dependent manner; a requirement for assembly of the prothrombinase complex.
• The first EGF homology domain contains a Ca+2 binding site which acts as a hinge to fold the EGF and GLA domains towards each other (12).
• This region of the molecule is involved in the recognition of cellular binding domains.
• Application:Suitable for RIA, ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 5ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.