85-7204-32　Factor VII (F.VII, Stable Factor, Proconvertin) 100ug　F0015

特徴

• Coagulation factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF) and is rapidly converted into VIIa.
• The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and also has important coagulation-independent functions such as angiogenesis.
• The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor.
• After cleavage of a signal peptide (residues 1 to 38), the mature chain can be further processed into the light chain (residues 39 to 190) and the heavy chain (residues 191 to 444).
• Applications:Suitable for RIA, ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: Coating: 5-10ug/ml; Secondary: 100-1000ug/ml
• Western Blot (Reduced and oxidized forms): 5ug/ml
• Optimal dilutions to be determined by the researcher.
• Extinction Coefficient:E1%, 1cm, 280nm = 14.0
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.