LFA-1 is a general leukocyte cell adhesion molecule, its activity being regulated by cell activation.
Hybridoma:Formed from the fusion of PAI mouse myeloma cells and LN cells from a BALB/c mouse.
Applications:Suitable for use in Flow Cytometry, Immunohistochemistry and Adhesion Blocking.
Other applications not tested.
Working Dilution:Flow Cytometry: 1:100-1:200 (0.5-1ug will label about 10e6 cells)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add glycerol (25–50%) and freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ug
Host：mouse
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Liquid in PBS, 0.02% sodium azide
Specificity：Recognizes the rat LFA-1 (lymphocyte function associated molecule-1) beta chain (CD18; 95-100kD) which is associated with the alpha chain (CD11a; 160-70kD). Therefore, this monoclonal antibody recognizes Mac-1 and gp150/95 as well as LFA-1. Inhibits homotypic aggregation of PHA blasts and blocks binding of rat lymphocytes to purified rat ICAM-1.
