85-7204-25　［取扱停止］RANTES (Regulated on Activation, Normal T-cell Expressed and Secreted) 500ug　R1100-11

特徴

• RANTES (regulated upon activation, normal T-cell expressed and secreted) is a 70-amino acid CC chemokine originally cloned from T cell-specific sequences-enriched cDNA libraries.
• Both human and murine RANTES are chemotactic for monocytes.
• Human RANTES is also a chemoattractant for T cells and eosinophils, as well as an activator of basophils for histamine release and of eosinophils.
• It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells.
• In contrast to other CC chemokines, RANTES is only slowly expressed after activation of leukocytes.
• It is involved in a number of pathological conditions like chronic inflammation and autoimmune diseases.
• Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency (SIV).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-1ug/ml.
• The detection limit under reducing conditions is 0.25-2.0ng/lane and under non-reducing conditions, 0.25-0.5ng/lane.
• ELISA (Sandwich): 2-4ug/ml in 100ul/well will detect at least 500pg/ml of recombinant human RANTES when used with R1100-03 at the detection antibody at 0.5-1ug/ml.
• Immunohistochemistry ((FFPE): 10ug/ml.
• Requires HIER with citrate buffer, pH 6.0
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.