Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines.
They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g.
macrophages, lymphocytes and NK cells.
While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes.
Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells.
Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells.
Applications:Suitable for use in ELISA, Neutralization and Western Blot.
Other applications have not been tested.
Recommended Dilutions:ELISA (Sandwich): 2-4ug/ml using 100ul/well antibody solution).
In conjunction with biotinylated anti-human Macrophage Inflammatory Protein 1 alpha (M1202-25C; 1-2ug/ml) as a detection antibody, allows the detection of at least 2000pg/ml of recombinant human Macrophage Inflammatory Protein 1 alpha.
Western Blot: 2-4ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human Macrophage Inflammatory Protein 1 alpha is 1-2ng/lane, under non-reducing conditions.
Neutralization: 5-10ug/ml yields one-half maximal inhibition of the biological activity of human Macrophage Inflammatory Protein 1 alpha (100ng/ml).
Optimal dilution determined by the researcher.
Hybridoma:Produced in Balb/c X ICR F1 mice.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a lyophilized powder. Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes human Macrophage Inflammatory Protein-1 alpha.
Clone # USB：8.PP.9
Isotype：IgG1,k
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human Macrophage Inflammatory Protein-1 alpha, expressed in E. coli.
