85-7204-06　Thyroid Stimulating Hormone (TSH) (HRP) 1mg　T5400-08A

特徴

• Thyroid-stimulating hormone (TSH or thyrotropin), produced in human pituitary glands, has a molecular mass of 25kD.
• An Important indicator of thyroid function, TSH is used to monitor thyroid associated diseases.
• Thyroid-stimulating hormone is a hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroid gland.
• TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4) and triiodothyronine (T3).
• TSH production is controlled by a Thyrotropin Releasing Hormone (TRH), which is manufactured in the hypothalamus and transported to the anterior pituitary gland, where it increases TSH production and release.
• Somatostatin is also produced by the hypothalamus, and has an opposite effect on the pituitary production of TSH, decreasing or inhibiting its release.
• The level of Thyroid hormones (T3 and T4) in the blood have an additional effect on the pituitary release of TSH.
• When the levels of T3 and T4 are low, the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased.
• This effect creates a regulatory negative feedback loop.
• TSH is a glycoprotein and consists of two subunits, the alpha and the beta subunit.
• The alpha subunit is identical to that of human chorionic gonadotropin (HCG), luteinizing hormone (LH), follicle-stimulating hormone (FSH).
• The beta subunit is unique to TSH, and therefore determines its function.
• TSH is a member of the cystine knot growth factor superfamily.
• It is a heterodimer of a 15kD unique subunit, TSH beta, with a 14kD alpha subunit, CGa (common glycoprotein hormone alpha) that is shared with lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG).
• Beta subunits of the four glycoprotein hormones share a 37-43% amino acid identity.
• Mature human TSH beta shares a 92%, 90%, 90%, 89%, 89%, 89%, and 88% aa identity with dog, rat, horse, mouse, cow, pig, and cat TSH b, respectively.
• Mature human CGa shares a 69%-73% aa identity with dog, rabbit, rat, mouse, cow, sheep, pig, cat and horse CGa.
• Each subunit forms a cystine knot structure with three disulfide bridges.
• A loop of the TSH beta subunit, termed a 'seat-belt', wraps around the CGa subunit to stabilize non-covalent association of the subunits, and also confers receptor selectivity.
• Structure and charge of the three N-linked carbohydrate chains influence activity; the most complex forms have lower activity but longer halflife.
• Bovine and porcine TSH bind human TSH receptors (TSHR) with high affinity.
• The hypothalamic peptide TRH stimulates production and secretion of TSH by thyrotrophs (basophile cells) in the anterior pituitary gland.
• TSH travels to thyroid TSHR to stimulate production of thyroxine (T4).
• In the tissues, T4 is converted to the active form of thyroid hormone, triiodothyronine (T3), which completes a feedback loop by inhibiting TSH production.
• Studies in mouse identify bone marrow as a secondary site of TSH production.
• In bone, TSH signaling through TSHR on osteoblast and osteoclast precursors negatively regulates skeletal remodeling.
• Bone marrow cells that produce TSH may also circulate to the thyroid and appear to modulate thyroid hormone activity in times of immunological stress.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Matched Pair:Suitable for use as detection or conjugate (label) antibody.
• Matched to T5400-22B as the capture or solid phase antibody.
• Recommended Dilution:Optimal dilution to be determined by the researcher.
• Affinity Constant: 2.1 x 10e10 L/mole
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.