Applications:Suitable for use in Immunohistochemistry and RIA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:200-1:500; Recognizes all well-recognized enkephalin immunoreactive sites but does not bind to areas known to contain beta-endorphin or dynorphin.
Optimal dilutions to be determined by the researcher.
Hybridoma:Secreted by a hybridoma formed by the fusion of a NSO/1 murine myeloma cell with a spleen cell from a BALB/c mouse immunized against Leu-enkephalin conjugated to bovine serum albumin.
Positive Control:Human small intestine
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：mouse
Source Antibody：human
Grade：Supernatant
Purity：Supernatant
Form：Supplied as a lyophilized powder with Thimersol. Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes human Met5- and Leu5- Enkephalin. Displays a 40% crossreactivity with C-terminal-extended Met-enkephalin hexapeptides and 7% crossreactivity with the extended heptapeptide.
