85-7203-35　Factor V 5x100ug　F0011-10

特徴

• Factor V is a large, single chain, plasma glycoprotein which is an essential component in the blood coagulation cascade.
• During coagulation, the procofactor, factor V, is converted to the active cofactor, factor Va, via limited proteolysis by the serine protease a-thrombin, and less efficiently by factor Xa.
• The active cofactor is composed of an NH2-terminal derived heavy chain (Mr=94,000) and a COOH-terminal derived light chain (Mr=74,000) which remain non-covalently associated in the presence of calcium ions.
• Factor Va serves as a cofactor for the serine protease factor Xa.
• Factor Va and Xa assemble on a phospholipid surface in a non-covalent and calcium ion-dependent manner, to form the prothrombinase complex.
• The prothrombinase complex is responsible for the rapid conversion of the zymogen prothrombin to the active serine protease, a-thrombin.
• Assembly of the prothrombinase complex increases the rate at which prothrombin is converted to thrombin by nearly 300,000-fold relative to the rate with factor Xa alone.
• Down regulation of the prothrombinase complex is accomplished partly through the inactivation of factor Va by activated protein C.
• Applications:Suitable for use in ELISA, RIA and Inhibitory Assays.
• Other applications have not been tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Kd: 3x10e-9M
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.