85-7202-93　［取扱停止］CD2 (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2, Human T & NK Cells) 200ug　C2253-10

特徴

• CD2 which is also known as E-rosette receptor, T11 and lymphocyte-function antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells.
• Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X).
• p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated after CD2 stimulation.
• CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis, apoptosis in activated peripheral T cells, and regulation of T cell anergy.
• Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells.
• The antigen has been characterized as the receptor for sheep erythrocytes and CD2 monoclonal antibodies that inhibit E rosette formation.
• CD2 interacts with lymphocyte function associated antigen (LFA3) and CD48/BCM1 to mediate adhesion between T cells and other cell types.
• CD2 is implicated in the triggerring of T cells, the cytoplasmic domain is implicated in the signaling function.
• It is useful for the identification of lymphomas and leukemias of T cell origin.
• Antigen distribution: T cells (E+) 100% Thymocytes 95 + 3% Peripheral blood lymphocytes 82 + 5% NK cells (CD16+) 50 + 10% Granulocytes < 1% Monocytes < 1% B Cells (E-) < 1%
• Applications:Suitable for use in ELISA.
• Useful for the detection of T and NK cells and classifying cases of Lymphoma.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:10,000
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 3 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.