The current monkeypox strain has approximately 50 genetic variations compared to related viruses that circulated in 2018-2019. While PCR methods are very accurate, they are vulnerable to genetic mutations as they only detect a short sequence within the genome. In contrast, serologic assays are less sensitive to mutational changes due to the high degree of antigenic relation among related virus strains and the polyclonal nature of the immune response.
Serologic assays provided significant diagnostic support during the 2003 U.S. monkeypox outbreak. Monkeypox virus-specific IgM is detectable within 2 days of rash onset and monkeypox IgM capture assays have been shown to distinguish between vaccinated and unvaccinated individuals with a sensitivity and specificity of 95% (doi:10.1128/CDLI.12.7.867-872.2005).
Represents a membrane protein which is a major target for neutralizing antibodies in humans.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：500ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：≥90% (SDS-PAGE). Affinity Chromatography
Form：Supplied as a liquid in 50mM Tris-HCl, 100mM sodium chloride, pH 8.0.
