85-6155-62　Membrane Cofactor Protein, Active, Recombinant, Human, hFc-Tag, aa35-328 (CD46) 100ug　587012

特徴

• Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
• Recombinant protein corresponding to aa35-328 from human Membrane cofactor protein, fused to hFc-Tag at C-terminal, expressed in Mammalian cells.
• Molecular Weight:~61.6kD
• Biological Activity:Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2ug/ml can bind Anti-CD46 rabbit monoclonal antibody, the EC50 of human CD46 protein is 0.8333-1.054ng/ml.
• Amino Acid Sequence:CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.